Simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in blood by GC-MS / 法医学杂志
Journal of Forensic Medicine
;
(6): 271-273, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-983663
ABSTRACT
OBJECTIVE@#To develop a method to measure trihexyphenidyl, chlorpromazine and clozapine in human blood with GC-MS.@*METHODS@#The specimens were alkalized (pH > 10) and extracted with V (benzene)V(ethyl acetate) = 11, and qualitatively analyzed using GC-MS-Full Scan with internal standard SKF525A. The specimens were alkalized (pH > 10) and extracted with V(benzene)V(ethyl acetate) = 11, and quantitatively analyzed using GC-MS-SIM with internal standard diazepam-d5.@*RESULTS@#The lowest detection limits of trihexyphenidyl, chlorpromazine and clozapine were 0.3, 0.3 and 0.7 ng/mL (S/N > or = 3) respectively. The calibration curve in 20-10 000 ng/mL showed a good linear distribution. The recovery rate was 79.9% to 85.5%. The RSDs of intraday and interday were less than 5.1%.@*CONCLUSION@#The established method was simple, sensitive and accurate for simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in human blood, and can be applied in forensic toxicological cases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Solvents
/
Antipsychotic Agents
/
Trihexyphenidyl
/
Chlorpromazine
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Clozapine
/
Forensic Toxicology
/
Hydrogen-Ion Concentration
/
Gas Chromatography-Mass Spectrometry
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2011
Type:
Article
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