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The Control of Drug Release and Vascular Endothelialization after Hyaluronic Acid-Coated Paclitaxel Multi-Layer Coating Stent Implantation in Porcine Coronary Restenosis Model
Korean Circulation Journal ; : 123-131, 2017.
Article in English | WPRIM | ID: wpr-98367
ABSTRACT
BACKGROUND AND

OBJECTIVES:

Hyaluronic acid (HA) is highly biocompatible with cells and the extracellular matrix. In contrast to degradation products of a synthetic polymer, degradation products of HA do not acidify the local environment. The aim of this study was to fabricate an HA-coated paclitaxel (PTX)-eluting stent via simple ionic interactions and to evaluate its effects in vitro and in vivo. MATERIALS AND

METHODS:

HA and catechol were conjugated by means of an activation agent, and then the stent was immersed in this solution (resulting in a HA-coated stent). After that, PTX was immobilized on the HA-coated stent (resulting in a hyaluronic acid-coated paclitaxel-eluting stent [H-PTX stent]). Study groups were divided into 4 groups bare metal stent (BMS), HA, H-PTX, and poly (L-lactide)-coated paclitaxel-eluting stent (P-PTX). Stents were randomly implanted in a porcine coronary artery. After 4 weeks, vessels surrounding the stents were isolated and subjected to various analyses.

RESULTS:

Smoothness of the surface was maintained after expansion of the stent. In contrast to a previous study on a PTX-eluting stent, in this study, the PTX was effectively released up to 14 days (a half amount of PTX in 4 days). The proliferation of smooth muscle cells was successfully inhibited (by 80.5±12.11% at 7 days of culture as compared to the control) by PTX released from the stent. Animal experiments showed that the H-PTX stent does not induce an obvious inflammatory response. Nevertheless, restenosis was clearly decreased in the H-PTX stent group (9.8±3.25%) compared to the bare-metal stent group (29.7±8.11%).

CONCLUSION:

A stent was stably coated with PTX via simple ionic interactions with HA. Restenosis was decreased in the H-PTX group. These results suggest that HA, a natural polymer, is suitable for fabrication of drug-eluting stents (without inflammation) as an alternative to a synthetic polymer.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Polymers / In Vitro Techniques / Stents / Paclitaxel / Coronary Vessels / Coronary Restenosis / Myocytes, Smooth Muscle / Animal Experimentation / Drug Evaluation, Preclinical / Extracellular Matrix Language: English Journal: Korean Circulation Journal Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Polymers / In Vitro Techniques / Stents / Paclitaxel / Coronary Vessels / Coronary Restenosis / Myocytes, Smooth Muscle / Animal Experimentation / Drug Evaluation, Preclinical / Extracellular Matrix Language: English Journal: Korean Circulation Journal Year: 2017 Type: Article