Analysis of the changes of amide A in rabbit heart after death by FTIR spectroscopy / 法医学杂志
Journal of Forensic Medicine
;
(6): 321-323, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-983671
ABSTRACT
OBJECTIVE@#To explore the relationship of changes of amide A in rabbit heart and the postmortem interval (PMI) by FTIR spectroscopy technique.@*METHODS@#Thirty-two rabbits were sacrificed and the hearts were sampled at 20 degrees C within 48 h postmortem points. All samples were sliced and tested by FTIR spectroscopy technique. The images of amide A were created by FTIR spectroscopic imaging. The positive and negative area ratios of amide A were analyzed using imaging analysis system.@*RESULTS@#The positive and negative area ratios declined regularly with the prolongation of death time in 48 h. There was a significant quadric relationship between the area ratios (y) of amide A (positive and negative area) and PMI(x). The regression equation was y = 0.001x2-0.038x + 0.747(R2 = 0.940).@*CONCLUSION@#The ratios of positive and negative area of amide A showed a strong correlation with PMI and could be used to estimate PMI.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Postmortem Changes
/
Time Factors
/
Regression Analysis
/
Spectroscopy, Fourier Transform Infrared
/
Forensic Pathology
/
Amides
/
Myocardium
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2011
Type:
Article
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