DNA extraction from decomposed tissue by double-digest and magnetic beads methods / 法医学杂志
Journal of Forensic Medicine
;
(6): 441-443, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-983697
ABSTRACT
OBJECTIVE@#To study the effect of the double-digest and magnetic beads method for DNA extraction from 3 types of decomposed tissues.@*METHODS@#DNA of cartilages, nails and joint capsule in 91 highly decomposed corpses which had not been extracted by common magnetic beads method, were prepared with the double-digest and magnetic beads methods, and quantified with Quantifiler kit, followed by amplification with Sinofiler kit or Minifiler kit.@*RESULTS@#DNA concentration extracted from the 91 highly decomposed cartilages, nails and joint capsule samples was 0-0.225 ng/microL. Sixty-two samples whose DNA concentration were more than 0.020 ng/microL had obtained 9 or more STR loci successfully. The detection rate was 68.13%.@*CONCLUSION@#The successful rate of STR genotyping for the 3 types of decomposed tissues can be significantly improved by the double-digest and magnetic beads methods.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reagent Kits, Diagnostic
/
Resins, Synthetic
/
Cadaver
/
DNA
/
Cartilage
/
Polymerase Chain Reaction
/
DNA Fingerprinting
/
Joint Capsule
/
Tandem Repeat Sequences
/
Forensic Medicine
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2011
Type:
Article
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