The number of circulating fibrocytes of skeletal muscle in rats after contusion / 法医学杂志
Journal of Forensic Medicine
; (6): 1-11, 2012.
Article
in Zh
| WPRIM
| ID: wpr-983702
Responsible library:
WPRO
ABSTRACT
OBJECTION@#To investigate the time-dependent appearance of circulating fibrocytes of skeletal muscle in rats after contusion.@*METHODS@#The model of skeletal muscle wound was established in rat. The circulating fibrocytes in contused skeletal muscle were detected by CD45 and procollagen I double immunofluorescence staining method.@*RESULTS@#In the control group, CD45- and procollagen I-positive cells were not detected in skeletal muscle. A few CD45 cells were observed aged from 6 h to 1 d after contusion. A few CD45- and procollagen I-positive cells (fibrocytes) initially gathered in injury area 3d after injury. The ratio of positive fibrocytes significantly increased 5 d after injury. The ratio of fibrocytes was highest at 7 d after contusion and then decreased. The volume of fibrocytes showed bigger with injury time increase compared with 3 d group. The expression of procollagen I and CD45 were weakened at 14d after injury.@*CONCLUSION@#The circulating fibrocytes are detected in contused skeletal muscle in time-dependent pattern. Circulating fibrocytes may be a marker in the wound age determination for contused skeletal muscle.
Full text:
1
Index:
WPRIM
Main subject:
Time Factors
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Wound Healing
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Immunohistochemistry
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Biomarkers
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Random Allocation
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Rats, Sprague-Dawley
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Leukocyte Common Antigens
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Muscle, Skeletal
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Contusions
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Collagen Type I
Limits:
Animals
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2012
Type:
Article