Changes of CK-MB and HSP 60 in electrical-injuried rats / 法医学杂志
Journal of Forensic Medicine
;
(6): 333-336, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-983756
ABSTRACT
OBJECTIVE@#To investigate the changes of creatine kinase-MB (CK-MB) and heat shock protein 60 (HSP 60) in rats without electric marks after electric injury, to identify the relationship of the CK-MB, HSP 60 and the time of electric injuries, and to evaluate the damage to cells after electric injury.@*METHODS@#The animal model of electric injury without electric marks was established by alternating current (voltage 110 V). Automatic biochemistry analyzer was used to detect the serum CK-MB and immunohistochemical staining technology was used to analyze the tissues of myocardium and left lobe of liver.@*RESULTS@#The amount of serum CK-MB was increased when the rats were injuried, and reached the peak at 30min. Then the amount of CK-MB began to decrease and showed a slight downward trend in 3-5 h after electric injury, and leveled off at 6 h. Immunohistochemistry staining also showed the changes of HSP 60 of rats' myocardial cells and hepatic cells regularly after electric injury.@*CONCLUSION@#The regular changes of serum CK-MB and tissular HSP 60 in rats can be used to diagnosis electric injury and assess the injury of internal organs after the electric injury without electric marks.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunohistochemistry
/
Chaperonin 60
/
Electric Injuries
/
Creatine Kinase, MB Form
/
Liver
/
Myocardium
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS