Distribution of deltamethrin in acute poisoned rats / 法医学杂志
Journal of Forensic Medicine
;
(6): 25-30, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-983787
ABSTRACT
OBJECTIVE@#To establish an animal model in acute poisoned rat by deltamethrin and an analysis method for determination of deltamethrin by gas chromatography-electron capture detector (GC-ECD) and to study the distribution of deltamethrin in rats in order to provide the references for forensic medicine identification about such cases.@*METHODS@#Rats were administered with deltamethrin of different doses(512 and 1,024 mg/kg) and killed 1.5 h later to be dissected rapidly for tissues (blood, hearts, livers, lungs, kidneys and brains etc.). Samples were dehydrated by anhydrous sodium sulfate and extracted with petroleum ether and acetone (VV=41). The level of deltamethrin was determined by GC-ECD.@*RESULTS@#There was a good separate between deltamethrin and endogenous impurities. The limit of quantification for deltamethrin in blood and liver were 0.1 microg/mL and 0.1 microg/g (S/N> or =10), respectively. The recovery rate of deltamethrin in blood was 91.55%-134.37% and both inter-day and intra-day precisions were less than 5.67%. The distribution of deltamethrin in poisoned rats with 512 mg/kg was as follow lungs > livers > hearts > kidneys > blood > brains and with 1 024 mg/kg dose was lungs > blood > hearts > kidneys > brains > livers (P<0.05).@*CONCLUSION@#The GC-ECD method is sensitive for determination of deltamethrin. The distribution of deltamethrin in rats has a dose-dependent manner. The study suggests that samples of blood, hearts, livers, lungs, kidneys and brains are suitable for deltamethrin poisoned analysis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pyrethrins
/
Tissue Distribution
/
Linear Models
/
Reproducibility of Results
/
Chromatography, Gas
/
Sensitivity and Specificity
/
Disease Models, Animal
/
Forensic Toxicology
/
Kidney
/
Liver
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2013
Type:
Article
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