Influences of cycle index and volume on sensitivity of DNA detection / 法医学杂志
Journal of Forensic Medicine
;
(6): 125-126, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-983806
ABSTRACT
OBJECTIVE@#To explore the feasibility of improving the sensitivity of DNA detection by increasing the PCR cycle index and decreasing the volume of amplifying system.@*METHODS@#The DNA of semen were collected from 10 healthy irrelevant volunteers, and were quantified to 50, 40, 30, 25, 20, 15, 10 pg/microL, separately. All samples were then amplified in 10, 5, 3 microL volume and at 28, 30, 32, 34, 36 cycles, respectively. 3130 genetic analyzer was used to detect 15 autosomal STR loci.@*RESULTS@#Under the situation of 28 cycles and 3 microL volume, samples which achieved > 40 pg/microL could be correctly typed. Under the situation of 10, 5, 3 microL volume, samples which achieved > 20 pg/microL could be correctly typed at 34 cycles. When increasing the index to 36 cycles, they could not be correctly typed because of the non-specific band.@*CONCLUSION@#DNA detecting sensitivity can be improved to a certain extent by increasing the cycle index and decreasing the volume of amplifying system.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Semen
/
DNA
/
Feasibility Studies
/
Polymerase Chain Reaction
/
DNA Fingerprinting
/
Sensitivity and Specificity
/
Tandem Repeat Sequences
/
Forensic Genetics
/
Limit of Detection
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2013
Type:
Article
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