Modified TRIzol method for RNA and DNA co-extraction from blood / 法医学杂志
Journal of Forensic Medicine
;
(6): 209-211, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-983824
ABSTRACT
OBJECTIVE@#To establish a new method for RNA and DNA co-extraction from the same sample by TRIzol reagent.@*METHODS@#After the aqueous phase which contained total RNA was removed by traditional TRIzol method, the values of pH of the interphase phase and organic phase were adjusted. The DNA was precipitated with ethanol and purified with DNA IQ system. The purified DNA was measured in quality and quantity. As the template, it was amplified and typed by PCR-STR. The data was compared with that extracted by traditional TRIzol method.@*RESULTS@#The DNA extracted by this modified method showed a better result of quality and quantity than that by traditional TRIzol method and a good STR typing.@*CONCLUSION@#The modified TRIzol method is advisable and reliable to simultaneously extract both DNA and RNA from the same sample. It could be used for individual identification and paternity testing to satisfy the need of forensic science.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenols
/
Reagent Kits, Diagnostic
/
Blood Chemical Analysis
/
DNA
/
RNA
/
Polymerase Chain Reaction
/
DNA Fingerprinting
/
Forensic Medicine
/
Guanidines
/
Hydrogen-Ion Concentration
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2013
Type:
Article
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