Nitric oxide mediated TNF-α, IL-1β gene expression in liver induced by crush injury of rat's soft tissues / 法医学杂志
Journal of Forensic Medicine
;
(6): 250-256, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-983911
ABSTRACT
OBJECTIVE@#To explore the effect of nitric oxide (NO) on the gene expression of hepatic TNF-α and IL-1β by crush injury of rat's soft tissues.@*METHODS@#Rats were randomly divided into sham group, crush group, crush+aminoguanidine (AG) group, and crush+L-arginine (L-Arg) group. Activities of ALT and AST as well as NO level in serum were measured. Gene expressions of TNF-α and IL-1β were detected with RT-PCR.@*RESULTS@#Obvious increase in TNF-α and IL-1β mRNA expression was detected in the crush group compared with the sham group (P<0.05). After pretreated L-Arg, expressions of TNF-α and IL-1β mRNA were markedly increased (P<0.05). After pretreated AG, those indices obviously decreased (P<0.05). Activities of ALT and AST enhanced and NO level increased in the crush group compared with the sham group (P<0.05). Pretreatment with L-Arg or AG led to substantial increased or reduced activities of ALT and AST as well as NO levels, respectively.@*CONCLUSION@#Endogenous NO mediated TNF-α, IL-1β mRNA up expression in liver induced by increased production of NO after crush injury of rat's soft tissues.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Wounds and Injuries
/
RNA, Messenger
/
Gene Expression
/
Tumor Necrosis Factor-alpha
/
Interleukin-1beta
/
Liver
/
Nitric Oxide
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2014
Type:
Article
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