Apoptosis in Lungs and Liver after Crush Injury of Hindlimbs in Rat / 法医学杂志
Journal of Forensic Medicine
;
(6): 88-92, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-983969
ABSTRACT
OBJECTIVE@#To investigate the process of apoptosis in lungs and liver induced by crushing hindlimbs of rat, and study the mechanism of crush injury.@*METHODS@#The rat experimental model of hindlimbs crush injury was established. The cell apoptosis in lungs and liver was detected by TUNEL assay, and the expression of Bax, Bcl-2 and caspase-3 apoptin was examined by immunohistochemistry.@*RESULTS@#Compared with the control group, the partial muscle injury of rat's hindlimbs was more serious with more apoptosis observed in lungs and liver (P < 0.05). The expression of Bax was up-regulated and Bcl-2 was down-regulated, whereas caspase-3 expression was activated (P < 0.05).@*CONCLUSION@#The cell apoptosis has increased significantly in lungs and liver after crush injury of hindlimbs in rat. The correlation factor released during tissue injury may mediate apoptosis process.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunohistochemistry
/
Up-Regulation
/
Apoptosis
/
Genes, bcl-2
/
Bcl-2-Associated X Protein
/
Caspase 3
/
Hindlimb
/
Liver
/
Lung
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2015
Type:
Article
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