Effects of PI3K/Akt Pathway in Wound Healing Process of Mice Skin / 法医学杂志
Journal of Forensic Medicine
;
(6): 7-12, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-984033
ABSTRACT
OBJECTIVE@#To investigate the expressions and time-dependent changes of phosphatidylinositol-3-kinase (PI3K), phospho-PI3K (p-PI3K), protein kinase B (PKB/Akt) and phospho-Akt (p-Akt) during wound healing process of mice skin.@*METHODS@#The changes of PI3K, p-PI3K, Akt and p-Akt expression in skin wound were detected by immunohistochemistry, Western blotting and real-time PCR.@*RESULTS@#Immunohistochemistry showed the expression of PI3K and p-Akt were observed in mononuclear and fibroblast after skin wound, and reached peak in reconstruction. The positive bands of PI3K, p-PI3K, Akt and p-Akt were observed in all time points of the wound healing process by Western blotting. The expression peak of p-PI3K and p-Akt showed in inflammation and proliferation; the expression peak of PI3K and Akt in reconstruction. Real-time PCR showed the expression peak of PI3K mRNA in inflammation and reconstruction and the peak of Akt mRNA in reconstruction.@*CONCLUSION@#During the wound healing process, the expressions of PI3K, Akt, p-PI3K and p-Akt show different changes with significant correlation to wound time. The expression of PI3K/Akt may be a valuable marker for wound time estimation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Skin
/
Wound Healing
/
Signal Transduction
/
Blotting, Western
/
Phosphatidylinositol 3-Kinases
/
Proto-Oncogene Proteins c-akt
/
Phosphatidylinositol 3-Kinase
/
Class I Phosphatidylinositol 3-Kinases
/
Real-Time Polymerase Chain Reaction
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2016
Type:
Article
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