Isolation and culture of neural stem cells in ventral midbrain of a rat embryo / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
;
(12): 394-396, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-984422
ABSTRACT
@#ObjectiveTo establish a method of isolation and culture of neural stem cells(NSCs). MethodsTissues of ventral midbrain were isolated from a rat embryo,and the NSCs were cultured stimulated with basic fibroblast growth factor(bFGF)in vitro.The cells were identified by immunocytochemistry.ResultsNSCs proliferated into neurosphere in symmetric and non-symmetric cleavage ways,and differentiated into neuron, astroglia and oligodendrosyte. ConclusionsThe method has been established to isolate and culture the neural stem cells.
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Rehabilitation Theory and Practice
Year:
2003
Type:
Article
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