Simultaneous Determination of Three Kinds of Effective Constituents in Cannabis Plants by Reversed-phase HPLC / 法医学杂志
Journal of Forensic Medicine
;
(6): 261-263, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-984843
ABSTRACT
OBJECTIVES@#To establish a high performance liquid chromatographic (HPLC) method for simultaneous determination of three effective constituents, including tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in Cannabis plants.@*METHODS@#A C₁₈ column was used in this study, and acetonitrile-phosphate buffer (0.015 mol/L KH₂PO₄) was used as mobile phase at a flow rate of 1.0 mL/min. At a detection wavelength of 220 mm, UV absorption spectra were collected at the wavelength range of 190-400 nm, and the spectra and retention time were counted as qualitative evidence.@*RESULTS@#THC, CBD and CBN could be well separated by this method. Three components had good linear relationship in the range of 0.4-40 μg/mL (R²≥0.999 3). The recoveries were over 87%. The limits of detection were 1.8 ng, 2.0 ng and 1.3 ng, respectively. The relative standard deviation (RSD) were less than 5% for both inter-day and intra-day precisions.@*CONCLUSIONS@#Reversed-phase HPLC method is simple, rapid and accurate, and it is suitable for the qualitative and quantitative detection of THC, CBD and CBN in Cannabis plants.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Dronabinol
/
Cannabidiol
/
Cannabinol
/
Cannabis
/
Chromatography, High Pressure Liquid
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2016
Type:
Article
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