Determination of the Content of 4-FMA in Rat Plasma Samples by HPLC-MS/MS Method / 法医学杂志
Journal of Forensic Medicine
;
(6): 419-422, 2019.
Article
in English
| WPRIM
| ID: wpr-985028
ABSTRACT
Objective To develop a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of the content of 4-fluoromethamphetamine (4-FMA) in rat plasma, and to provide a methodological basis for the study of the toxicokinetics of 4-FMA in rats. Methods Rat plasma samples were added into internal standard methamphetamine (MA). Its proteins were precipitated with methanol and then separated with Poroshell 120 EC-C18 chromatographic column. A 0.1% formic acid aqueous solution and a 0.1% formic acid acetonitrile solution were used as the mobile phase at the flow rate of 0.4 mL/min. Electrospray ionization source was used for detection in the multiple reaction monitoring (MRM) mode. Results The linear relationship was good when the mass concentration of 4-FMA in plasma samples was in the range of 5-1 000 ng/mL (r>0.999). The limit of detection (LOD) was 3 ng/mL and the limit of quantification (LOQ) was 5 ng/mL. The accuracy was expressed as relative error (RE), and in the range of ±5%, the intra-day precision and inter-day precision (relative standard deviation, RSD) less than 9%, and the extraction recovery rate was more than 90%. The analysis and detection of plasma samples were completed within 2.5 min. Conclusion This study developed a HPLC-MS/MS method for the determination of 4-FMA in rat plasma samples. This method is accurate, rapid, simple and sensitive and can be applied to the study of toxicokinetics of 4-FMA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Tandem Mass Spectrometry
/
Limit of Detection
/
Toxicokinetics
/
Methamphetamine
Limits:
Animals
Language:
English
Journal:
Journal of Forensic Medicine
Year:
2019
Type:
Article
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