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Changes of AP-1 Binding Activities in Rat Brain by Electroconvulsive Shock / 신경정신의학
Journal of Korean Neuropsychiatric Association ; : 544-552, 2003.
Article in Korean | WPRIM | ID: wpr-9869
ABSTRACT

OBJECTIVES:

ECS could have therapeutic effects on psychiatric illnesses by inducing IEGs, which in turn regulates expression of their target genes. We observed AP-1 binding activity and identified AP-1 binding proteins in NMDAR1, late response gene of IEGs, which considered as the candidate gene for schizophrenia.

METHODS:

By gel shift assay and supershift assay, we observed binding activities and AP-1 binding proteins in NMDAR1. Because IEGs are induced rapidly but transiently by external stimuli, there is a possibility that the expression of IEGs is negatively feedbacked by their own products via their AP-1 binding sites. For that purpose, we also observed AP-1 binding activity of c-fos and c-jun via gel shift and supershift assay.

RESULTS:

ECS increased AP-1 binding activities of NMDAR1 gene, contributed by c-Fos and its related proteins. Peak of the increased binding was 60 minutes in both hippocampus and cerebellum. Though expression of c-Fos and c-Jun were increased by ECS, there were no changes in AP-1 binding activities after ECS. AP-1 sites of IEGs were binded by CREB, regardless of ECS.

CONCLUSION:

There is a possibility that ECS induced IEG expression, and then incresed expression of NMDR1 by binding of expressed IEGs to the AP-1 site of NMDAR1. ECS did not increase AP-1 binding activities of IEGs. This suggests that the regulation of IEGs' expression can not be influenced mainly by AP-1 site.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Schizophrenia / Binding Sites / Brain / Carrier Proteins / Cerebellum / Transcription Factor AP-1 / Electroshock / Hippocampus Type of study: Prognostic study Limits: Animals Language: Korean Journal: Journal of Korean Neuropsychiatric Association Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Schizophrenia / Binding Sites / Brain / Carrier Proteins / Cerebellum / Transcription Factor AP-1 / Electroshock / Hippocampus Type of study: Prognostic study Limits: Animals Language: Korean Journal: Journal of Korean Neuropsychiatric Association Year: 2003 Type: Article