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Clinical Efficacy Evaluation of Multi-parameter Real-time Polymerase Chain Reaction for the Central Venous Catheter-related Blood Stream Infection / 감염과화학요법
Infection and Chemotherapy ; : 240-244, 2011.
Article in Korean | WPRIM | ID: wpr-9932
ABSTRACT

BACKGROUND:

The study evaluated the clinical efficacy of a multi-parameter real-time polymerase chain reaction (PCR) test for patients with central venous catheter-related bloodstream infection (CRBSI). MATERIALS AND

METHODS:

Thirty five patients suspected to have CRBSI were enrolled. The SeptiFast(TM) (SF) multi-parameter real-time PCR test (Roche Diagnostics, Germany) and blood culture were performed and results were compared.

RESULTS:

The turn-around time for the SF test and blood culture was 32.6+/-28.9 hours and 115.8+/-23.5 hours, respectively. Among the 70 blood samples, the positive rates of SF test and blood culture were 34.3% and 27.1%, respectively, and the agreement rate was 62.9%. Gram-positive bacteria were detected in 10 patients with blood culture and 11 patients with SF test. Gram-negative bacteria were detected in one patient with a blood culture and in seven patients with SF test. Candida was not detected in blood culture but was detected in two patients by the SF test.

CONCLUSIONS:

SF test was faster and more sensitive for the detection of blood pathogens than blood culture. It provides a more sensitive detection of gram-negative and Candida in blood than does blood culture testing.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Candida / Rivers / Catheter-Related Infections / Real-Time Polymerase Chain Reaction / Gram-Negative Bacteria / Gram-Positive Bacteria Limits: Humans Language: Korean Journal: Infection and Chemotherapy Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Candida / Rivers / Catheter-Related Infections / Real-Time Polymerase Chain Reaction / Gram-Negative Bacteria / Gram-Positive Bacteria Limits: Humans Language: Korean Journal: Infection and Chemotherapy Year: 2011 Type: Article