Clinical Efficacy Evaluation of Multi-parameter Real-time Polymerase Chain Reaction for the Central Venous Catheter-related Blood Stream Infection / 감염과화학요법
Infection and Chemotherapy
;
: 240-244, 2011.
Article
in Korean
| WPRIM
| ID: wpr-9932
ABSTRACT
BACKGROUND:
The study evaluated the clinical efficacy of a multi-parameter real-time polymerase chain reaction (PCR) test for patients with central venous catheter-related bloodstream infection (CRBSI). MATERIALS ANDMETHODS:
Thirty five patients suspected to have CRBSI were enrolled. The SeptiFast(TM) (SF) multi-parameter real-time PCR test (Roche Diagnostics, Germany) and blood culture were performed and results were compared.RESULTS:
The turn-around time for the SF test and blood culture was 32.6+/-28.9 hours and 115.8+/-23.5 hours, respectively. Among the 70 blood samples, the positive rates of SF test and blood culture were 34.3% and 27.1%, respectively, and the agreement rate was 62.9%. Gram-positive bacteria were detected in 10 patients with blood culture and 11 patients with SF test. Gram-negative bacteria were detected in one patient with a blood culture and in seven patients with SF test. Candida was not detected in blood culture but was detected in two patients by the SF test.CONCLUSIONS:
SF test was faster and more sensitive for the detection of blood pathogens than blood culture. It provides a more sensitive detection of gram-negative and Candida in blood than does blood culture testing.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Candida
/
Rivers
/
Catheter-Related Infections
/
Real-Time Polymerase Chain Reaction
/
Gram-Negative Bacteria
/
Gram-Positive Bacteria
Limits:
Humans
Language:
Korean
Journal:
Infection and Chemotherapy
Year:
2011
Type:
Article
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