Strain-specific PCR Primers for the Detection of Prevotella intermedia ATCC 49046
International Journal of Oral Biology
;
: 79-82, 2011.
Article
in English
| WPRIM
| ID: wpr-9938
ABSTRACT
The aim of this study was to develop Prevotella intermedia ATCC 49046-specific PCR primers designed based on the nucleotide sequence of a DNA probe Pig28. The strain-specificity of the PCR primers, Pig28-F1/Pig28-R1, was confirmed with 9 strains of P. intermedia and 25 strains (15 species) of Prevotella species. The detection limit of the PCR primers was 2 pg of the purified genomic DNA of P. intermedia ATCC 49046. These PCR primers were found to be useful for identifying P. intermedia ATCC 49046, particularly for determining the authenticity of the strain.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sprains and Strains
/
DNA
/
Base Sequence
/
Polymerase Chain Reaction
/
Prevotella intermedia
/
Prevotella
/
Limit of Detection
Type of study:
Diagnostic study
Language:
English
Journal:
International Journal of Oral Biology
Year:
2011
Type:
Article
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