The Inhibition of Human Telomerase Reverse Transcriptase Expression by Peroxiredoxin I and c-Myc in Prostatic Cancer Cells / 대한비뇨기과학회지
Korean Journal of Urology
;
: 418-425, 2006.
Article
in Korean
| WPRIM
| ID: wpr-99395
ABSTRACT
PURPOSE:
We evaluated the hypothesis that the telomerase expression is associated with c-Myc and peroxiredoxin I (Prx I) in patients with prostate cancer. The study determined the link between Prx I, c-Myc and human telomerase reverse transcriptase (hTERT) in prostate cancer cells. MATERIALS ANDMETHODS:
The cDNA of the Prx I gene was obtained by reverse-transcriptase polymerase chain reaction (RT-PCR) amplification. Cotransfections were performed by using a hTERT luciferase reporter plasmid and each expression vector as indicated (c-Myc or Prx I). Empty vectors were used as controls for determining the basal promoter activity. RT-PCR was performed to evaluate the effect of the DEM-induced Prx I mRNA expression. Luciferase assay was performed to evaluate the inhibitory effect of transfected Prx I and the DEM induced Prx I on the transcriptional activity of hTERT in the human prostatic cancer cell lines PC-3 and DU-145.RESULTS:
In this study, we found that Prx I could inhibit hTERT expression through direct interaction with c-Myc protein in the prostate cancer cell lines. In addition, it was obvious that Prx I could interact with c-Myc protein. We also found that DEM could induce upregulation of the Prx I mRNA expression and that the increased expression of Prx I could downregulate the expression of hTERT.CONCLUSIONS:
Our results demonstrated a direct link between Prx I, c-Myc and hTERT, and we suggest that Prx I regulates cellular immortalization through c-Myc and hTERT, which is activation step in carcinogenesis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Prostatic Neoplasms
/
RNA, Messenger
/
Up-Regulation
/
Cell Line
/
Polymerase Chain Reaction
/
DNA, Complementary
/
Telomerase
/
Peroxiredoxins
/
Carcinogenesis
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Urology
Year:
2006
Type:
Article
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