The change in phospholipase C-gamma1 expression following balloon injury to the rat carotid artery / 대한내과학회지
Korean Journal of Medicine
;
: 234-241, 2001.
Article
in Korean
| WPRIM
| ID: wpr-99489
ABSTRACT
BACKGROUND:
While inositol phospholipid-specific phospholipase C (PLC) plays a central role in signal transduction pathways, little is known about its role in the vascular response to injury. Recent studies have shown that phospholipase C-gamma1 (PLC-gamma1) is required for PDGF-induced DNA synthesis and angiotensin II signaling. This study was undertaken to determine the potential involvement of PLC-gamma1 in the in vivo response to vascular injury.METHODS:
Vascular injury was achieved in the left common carotid artery of six-month-old male Wistar rats. The expression of PLC-gamma1 was evaluated at serial time points by immunohistochemistry and Western blot analysis following balloon de-endothelialization of the rat carotid artery.RESULTS:
In the denuded carotid artery at 1 week, the neointima became thicker in a symmetrical manner with respect to the long axis. A strong expression of PLC-gamma1 at one week after injury was seen primarily in the thin layers of neointima. This increased immunoreactivity of PLC-gamma1 persisted at 2-3 weeks after injury, coinciding with the time when neointima gains of its mass. At 4 weeks after injury, staining intensity slightly declined but levels remained elevated. As determined by Western blot analysis, the amount of PLC-gamma1 was about 3-fold higher at 3 weeks after injury compared to uninjured vessels (p<0.01).CONCLUSION:
These results suggest that the amplification of traffic within signal transduction pathways involving PLC-gamma1 occurs and may play a significant role in neointima formation following arterial injury.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Type C Phospholipases
/
Phospholipases
/
Axis, Cervical Vertebra
/
DNA
/
Angiotensin II
/
Immunohistochemistry
/
Carotid Arteries
/
Signal Transduction
/
Blotting, Western
/
Rats, Wistar
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Korean Journal of Medicine
Year:
2001
Type:
Article
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