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The Effect of Umbilical Cord Blood Derived Mesenchymal Stem Cells in Monocrotaline-induced Pulmonary Artery Hypertension Rats
Journal of Korean Medical Science ; : 576-585, 2015.
Article in English | WPRIM | ID: wpr-99850
ABSTRACT
Pulmonary arterial hypertension (PAH) causes right ventricular failure due to a gradual increase in pulmonary vascular resistance. The purposes of this study were to confirm the engraftment of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) placed in the correct place in the lung and research on changes of hemodynamics, pulmonary pathology, immunomodulation and several gene expressions in monocrotaline (MCT)-induced PAH rat models after hUCB-MSCs transfusion. The rats were grouped as follows the control (C) group; the M group (MCT 60 mg/kg); the U group (hUCB-MSCs transfusion). They received transfusions via the external jugular vein a week after MCT injection. The mean right ventricular pressure (RVP) was significantly reduced in the U group after the 2 week. The indicators of RV hypertrophy were significantly reduced in the U group at week 4. Reduced medial wall thickness in the pulmonary arteriole was noted in the U group at week 4. Reduced number of intra-acinar muscular pulmonary arteries was observed in the U group after 2 week. Protein expressions such as endothelin (ET)-1, endothelin receptor A (ERA), endothelial nitric oxide synthase (eNOS) and matrix metalloproteinase (MMP)-2 significantly decreased at week 4. The decreased levels of ERA, eNOS and MMP-2 immunoreactivity were noted by immnohistochemical staining. After hUCB-MSCs were administered, there were the improvement of RVH and mean RVP. Reductions in several protein expressions and immunomodulation were also detected. It is suggested that hUCB-MSCs may be a promising therapeutic option for PAH.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pulmonary Artery / Immunohistochemistry / Gene Expression Regulation / Cytokines / Monocrotaline / Rats, Sprague-Dawley / Hypertrophy, Right Ventricular / Endothelin-1 / Matrix Metalloproteinase 2 / Receptor, Endothelin A Limits: Animals / Humans / Male Language: English Journal: Journal of Korean Medical Science Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pulmonary Artery / Immunohistochemistry / Gene Expression Regulation / Cytokines / Monocrotaline / Rats, Sprague-Dawley / Hypertrophy, Right Ventricular / Endothelin-1 / Matrix Metalloproteinase 2 / Receptor, Endothelin A Limits: Animals / Humans / Male Language: English Journal: Journal of Korean Medical Science Year: 2015 Type: Article