Técnica de diafanización para describir el desarrollo embrionario del sistema óseo. Revisión de la literatura / Diaphanization technique for the description of the embryonic development of the skeletal system. Systematic literature review
Univ. med
; 57(4): 488-501, oct. - dic. 2016. ilus, tab
Article
en Es
| LILACS, COLNAL
| ID: biblio-1007307
Biblioteca responsable:
CO185.1
ABSTRACT
Introduction:
The anatomic diaphanization technique consists in the transparency of soft tissues of vertebrate organisms (clearing), in order to dye the mineralized tissue and visualize bone and cartilage (staining).Objective:
To review the reports available on specialized literature that describes the embryonic development of mammal Ìs skeletal system through the diaphonization technique and simple staining or double staining processes. Materials andmethods:
A literature review was made on PubMed, Academic Google and SciELO, based on PRISMA, through the association of the term double staining with the health descriptors of the Medical Subject Headings (MeSH) alizarin red, alician blue, bone and cartilage , and the combination of the Boolean operators + and &.Results:
There were included 22 articles that described the diaphanization technique and the simple staining or double staining technique used in the observation, registration and analysis of the embryonic development of mammal Ìs skeletal system.Conclusion:
The diaphanization technique and simple staining or double staining technique is an anatomic study techniqueversatile and of low costof the embryonic development of mammal Ìs skeletal system, which may be used in toxicology studies, discarding the possibility of developmental anomalies during the cartilage or bone formation (chondrogenesis and osteogenesis, respectively) associated to the exposure to a possible teratogenic agent.Palabras clave
Texto completo:
1
Índice:
LILACS
Asunto principal:
Anatomía Comparada
Tipo de estudio:
Systematic_reviews
Límite:
Humans
Idioma:
Es
Revista:
Univ. med
/
Universitas Médica
Asunto de la revista:
MEDICINA
/
SAUDE PUBLICA
Año:
2016
Tipo del documento:
Article