Genotyping of toxoplasma gondii: DNA extraction from formalin-fixed paraffin-embedded autopsy tissues from AIDS patients who died by severe disseminated toxoplasmosis
Experimental parasitology
;
: 1-24, 2016. tab
Artículo
en Inglés
| LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-IALPROD, SES-SP
| ID: biblio-1009200
ABSTRACT
This study investigated the genetic features of T. gondii isolated directly in autopsies of HIV-infected patients who died with severe disseminated toxoplasmosis. This retrospective analysis was conducted in a cohort of 15 HIV-infected patients with clinical and laboratory data. They had previous cerebral toxoplasmosis at least 6 months before the disseminated toxoplasmosis episode. The hypothesis was that they were infected with highly virulent parasites due to the condition in which they died. T. gondii genotyping was done directly in DNA extracted from 30 autopsy brain and lung samples (2 per patient) and mutilocus PCR-RFLP genotyping was done using 12 molecular markers. The 30 clinical samples were genotyped successfully in 8 or more loci and six suggestive genotypes were identified. One of them was Toxo DB #11, previously identified in different domestic animals and virulent in experimental animals. The other five suggestive genotypes...(AU)
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Toxoplasma
/
Síndrome de Inmunodeficiencia Adquirida
/
ADN de Forma A
Idioma:
Inglés
Revista:
Experimental parasitology
Año:
2016
Tipo del documento:
Artículo
Institución/País de afiliación:
Secretaria da Saúde do Estado de São Paulo. Instituto Adolfo Lutz/BR
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