Chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in C57BL/6 mice
Biol. Res
;
52: 30, 2019. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1011432
ABSTRACT
BACKGROUND:
Chronic prostatitis has been supposed to be associated with preneoplastic lesions and cancer development. The objective of this study was to examine how chronic inflammation results in a prostatic microenvironment and gene mutation in C57BL/6 mice.METHODS:
Immune and bacterial prostatitis mouse models were created through abdominal subcutaneous injection of rat prostate extract protein immunization (EAP group) or transurethral instillation of uropathogenic E. coli 1677 (E. coli group). Prostate histology, serum cytokine level, and genome-wide exome (GWE) sequences were examined 1, 3, and 6 months after immunization or injection.RESULT:
In the EAP and E. coli groups, immune cell infiltrations were observed in the first and last months of the entire experiment. After 3 months, obvious proliferative inflammatory atrophy (PIA) and prostatic intraepithelial neoplasia (PIN) were observed accompanied with fibrosis hyperplasia in stroma. The decrease in basal cells (Cytokeratin (CK) 5+/p63+) and the accumulation of luminal epithelial cells (CK8+) in the PIA or PIN area indicated that the basal cells were damaged or transformed into different luminal cells. Hic1, Zfp148, and Mfge8 gene mutations were detected in chronic prostatitis somatic cells.CONCLUSION:
Chronic prostatitis induced by prostate extract protein immunization or E. coli infection caused a reactive prostatic inflammation microenvironment and resulted in tissue damage, aberrant atrophy, hyperplasia, and somatic genome mutation.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Lesiones Precancerosas
/
Prostatitis
/
Infecciones por Escherichia coli
/
Mutación
Límite:
Animales
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2019
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
The Affiliated Tumor Hospital of Guangxi Medical University/CN
/
The First Affiliated Hospital of Guangxi Medical University/CN
Similares
MEDLINE
...
LILACS
LIS