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Methylation status of the PPP1R13L promoter region among lung cancer patients and healthy controls. Analytical cross-sectional study
Yin, Jiaoyang; Ma, Yegang; Vogel, Ulla; Wang, Chunhong; Zhang, Ying; Wang, Huiwen.
  • Yin, Jiaoyang; Shenyang Medical College. Liaoning Education Ministry. Key Laboratory of Environment and Population Health. Shenyang. CN
  • Ma, Yegang; Liaoning Cancer Hospital. Department of Thoracic Surgery. Shenyang. CN
  • Vogel, Ulla; National Research Centre for the Working Environment. Copenhagen DK-2100. DK
  • Wang, Chunhong; Shenyang Medical College. Liaoning Education Ministry. Key Laboratory of Environment and Population Health. Shenyang. CN
  • Zhang, Ying; Shenyang Medical College. Liaoning Education Ministry. Key Laboratory of Environment and Population Health. Shenyang. CN
  • Wang, Huiwen; Shenyang Medical College. Liaoning Education Ministry. Key Laboratory of Environment and Population Health. Shenyang. CN
São Paulo med. j ; 137(3): 255-261, May-June 2019. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1020958
ABSTRACT
ABSTRACT

BACKGROUND:

There is evidence that genetic predisposition and epigenetic alteration (e.g. DNA methylation) play major roles in lung cancer. In our genetic epidemiological studies, rs1970764 in oncogene PPP1R13L was most consistently associated with lung cancer risk. Here, we explored the role of PPP1R13L methylation in lung cancer development. DESIGN AND

SETTING:

Analytical cross-sectional study (45 lung cancer cases and 45 controls), conducted in China.

METHODS:

We investigated the DNA methylation status of 2,160 cytosine-phosphate-guanine (CpG) sites in the PPP1R13L promoter region using the EpiTYPER assay of the Sequenom MassARRAY platform.

RESULTS:

In the whole study group, the methylation levels of CpG-6, CpG-9, CpG-20 and CpG-21 were significantly lower and those of CpG-16 were significantly higher in cases than in controls. Among smokers, the methylation levels at five CpG sites (CpG-6, CpG-11, CpG-15, CpG-20 and CpG-21) were statistically significantly lower among cases. Among men, the methylation levels at four CpG sites (CpG-11, CpG-15, CpG-20 and CpG-21) were significantly lower among cases. Regarding smokers, the methylation levels at CpG-7.8 and CpG-21 among cases and at CpG-22 among controls were significantly lower, compared with nonsmokers. The frequency of positivity for methylation was not significantly different between lung cancer cases and controls (68.22% for cases and 71.87% for controls; P = 0.119).

CONCLUSION:

Our study on a Chinese population suggests that lung cancer patients have aberrant methylation status (hypomethylation tended to be more frequent) in peripheral blood leukocytes at several CpG sites in the PPP1R13L promoter region and that exposure to smoking may influence methylation status.
Asunto(s)


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Proteínas Represoras / Metilación de ADN / Predisposición Genética a la Enfermedad / Péptidos y Proteínas de Señalización Intracelular / Neoplasias Pulmonares Tipo de estudio: Estudio observacional / Estudio de prevalencia / Factores de riesgo Límite: Anciano / Femenino / Humanos / Masculino Idioma: Inglés Revista: São Paulo med. j Asunto de la revista: Cirurgia Geral / Ciˆncia / Ginecologia / Medicina / Medicina Interna / Obstetr¡cia / Pediatria / Sa£de Mental / Sa£de P£blica Año: 2019 Tipo del documento: Artículo País de afiliación: China / Dinamarca Institución/País de afiliación: Liaoning Cancer Hospital/CN / National Research Centre for the Working Environment/DK / Shenyang Medical College/CN

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Proteínas Represoras / Metilación de ADN / Predisposición Genética a la Enfermedad / Péptidos y Proteínas de Señalización Intracelular / Neoplasias Pulmonares Tipo de estudio: Estudio observacional / Estudio de prevalencia / Factores de riesgo Límite: Anciano / Femenino / Humanos / Masculino Idioma: Inglés Revista: São Paulo med. j Asunto de la revista: Cirurgia Geral / Ciˆncia / Ginecologia / Medicina / Medicina Interna / Obstetr¡cia / Pediatria / Sa£de Mental / Sa£de P£blica Año: 2019 Tipo del documento: Artículo País de afiliación: China / Dinamarca Institución/País de afiliación: Liaoning Cancer Hospital/CN / National Research Centre for the Working Environment/DK / Shenyang Medical College/CN