Isolation and identification of allergens and biogenic amines of Prosopis juliflora genotypes
Electron. j. biotechnol
;
30: 24-32, nov. 2017. tab, ilus, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1021325
ABSTRACT
Background:
Prosopis, or mesquite (Prosopis juliflora (Sw.) DC.), was introduced in Saudi Arabia several decades ago and is heavily used in street, roadside, and park plantations. It shows great adaptation to the prevailing climatic conditions such as high temperature, severe drought, and salinity and spreads naturally in many parts of the Kingdom. This research was conducted to isolate allergen proteins and biogenic amines from the pollen grains of P. juliflora genotypes in Saudi Arabia from two regions, namely Al-Qassim and Eastern regions.Results:
The results showed that 18 different allergen proteins were detected in P. juliflora genotypes, with molecular weight ranging from 14 to 97 kDa. Moreover, P. juliflora genotypes from the two studied regions contained eight biogenic amines, namely histamine, tyramine, tryptamine, ß-phenylethylamine, butricine, codapherine, spermidine, and spermine. All genotypes from the Al-Qassim region were found to contain all eight amines, while in the Eastern region, histamine was absent in three genotypes, spermine was absent in six genotypes, and spermidine was absent in three genotypes. Genotypes B23, E20, and E21 had the lowest biogenic amine quantity.Conclusions:
All identified proteins from mesquite trees from both regions (Eastern and Al-Qassim) cause allergies in patients who are sensitive to pollen grains. Bioamines, except histamine and tyramine, were recorded at varying concentrations in different genotypes.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Polen
/
Aminas Biogénicas
/
Alérgenos
/
Prosopis
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2017
Tipo del documento:
Artículo
País de afiliación:
Egipto
/
Arabia Saudita
Institución/País de afiliación:
National Research Centre/EG
/
Qassim University/SA
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