Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
Mem. Inst. Oswaldo Cruz
; 112(2): 155-159, Feb. 2017. tab, graf
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| ID: biblio-1040566
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ABSTRACT
BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays. FINDINGS Our results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin. MAIN CONCLUSION Transfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells.
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Texto completo:
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Índice:
LILACS
Asunto principal:
Leishmania braziliensis
/
Anfotericina B
/
Proteínas Fluorescentes Verdes
/
Evaluación Preclínica de Medicamentos
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Antiprotozoarios
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Año:
2017
Tipo del documento:
Article