Identification of a type I nitroreductase gene in non-virulent Trypanosoma rangeli
Mem. Inst. Oswaldo Cruz
;
112(7): 504-509, July 2017. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1040572
ABSTRACT
ABSTRACT Trypanosomatid type I nitroreductases (NTRs), i.e., mitochondrial enzymes that metabolise nitroaromatic pro-drugs, are essential for parasite growth, infection, and survival. Here, a type I NTR of non-virulent protozoan Trypanosoma rangeli is described and compared to those of other trypanosomatids. The NTR gene was isolated from KP1(+) and KP1(-) strains, and its corresponding transcript and 5' untranslated region (5'UTR) were determined. Bioinformatics analyses and nitro-drug activation assays were also performed. The results indicated that the type I NTR gene is present in both KP1(-) and KP1(+) strains, with 98% identity. However, the predicted subcellular localisation of the protein differed among the strains (predicted as mitochondrial in the KP1(+) strain). Comparisons of the domains and 3D structures of the NTRs with those of orthologs demonstrated that the nitroreductase domain of T. rangeli NTR is conserved across all the strains, including the residues involved in the interaction with the FMN cofactor and in the tertiary structure characteristics of this oxidoreductase protein family. mRNA processing and expression were also observed. In addition, T. rangeli was shown to be sensitive to benznidazole and nifurtimox in a concentration-dependent manner. In summary, T. rangeli appears to have a newly discovered functional type I NTR.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Nitrorreductasas
/
Trypanosoma rangeli
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2017
Tipo del documento:
Artículo
País de afiliación:
Chile
/
Colombia
/
España
Institución/País de afiliación:
Consejo Superior de Investigaciones Científicas/ES
/
Pontificia Universidad Católica de Valparaíso/CL
/
Pontificia Universidad Javeriana/CO
Similares
MEDLINE
...
LILACS
LIS