Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin
Mem. Inst. Oswaldo Cruz
;
114: e180529, 2019. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1040608
ABSTRACT
Helmintex is a sensitive method used for detecting Schistosoma mansoni eggs. Here, we describe the observed frequency of six proposed criteria associated with the identification of S. mansoni eggs prepared with the Helmintex method and stained with ninhydrin. The efficacy of these criteria in classifying S. mansoni eggs when applied in various combinations was also examined. Nine observers registered the presence or absence of 6 different criteria in 100 eggs using a microscope at 100x magnification. Ninhydrin purple, which was frequently observed, was the criterion associated with the lowest inter-observer variability. At least three criteria were associated with a significantly better performance in egg identification. In conclusion, ninhydrin staining and a combination of criteria are recommended for microscope examination of faecal sediments.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Óvulo
/
Recuento de Huevos de Parásitos
/
Schistosoma mansoni
/
Heces
/
Indicadores y Reactivos
/
Ninhidrina
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2019
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Pontifícia Universidade Católica do Rio Grande do Sul/BR
Similares
MEDLINE
...
LILACS
LIS