A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
Mem. Inst. Oswaldo Cruz
;
114: e190158, 2019. graf
Artículo
en Inglés
| LILACS
| ID: biblio-1040629
ABSTRACT
As phagocytosis is the first line of defense against malaria, we developed a phagocytosis assay with Plasmodium vivax (P. vivax) merozoites that can be applied to evaluate vaccine candidates. Briefly, after leukocyte removal with loosely packed cellulose powder in a syringe, P. vivax trophozoites matured to the merozoite-rich schizont stages in the presence of the E64 protease inhibitor. The Percoll gradient-enriched schizonts were chemically disrupted to release merozoites that were submitted to merozoite opsonin-dependent phagocytosis in two phagocytic lines with human and mouse antibodies against the N- and C-terminus of P. vivax Merozoite Surface Protein-1 (Nterm-PvMSP1 and MSP119). The resulting assay is simple and efficient for use as a routine phagocytic assay for the evaluation of merozoite stage vaccine candidates.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Fagocitosis
/
Plasmodium vivax
/
Anticuerpos Antiprotozoarios
/
Proteínas Protozoarias
/
Merozoítos
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2019
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Fundação Oswaldo Cruz-Fiocruz/BR
/
Fundação de Medicina Tropical Dr Heitor Vieira Dourado/BR
/
Universidade Federal do Amazonas/BR
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