A duplex endpoint PCR assay for rapid detection and differentiation of leptospira strains
Rev. Soc. Bras. Med. Trop
;
50(2): 239-242, Mar.-Apr. 2017. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1041406
ABSTRACT
Abstract INTRODUCTION:
This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains.METHODS:
Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively.RESULTS:
The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water.CONCLUSIONS:
Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
ADN Bacteriano
/
Reacción en Cadena de la Polimerasa
/
Cartilla de ADN
/
Leptospira
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
Medicina Tropical
Año:
2017
Tipo del documento:
Artículo
País de afiliación:
Malasia
/
Reino Unido
Institución/País de afiliación:
Ministry of Health/MY
/
University of London/GB
/
University of Malaya/MY
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