Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR
Rev. Soc. Bras. Med. Trop
;
51(4): 528-532, July-Aug. 2018. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1041475
ABSTRACT
Abstract INTRODUCTION Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. METHODS This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. RESULTS: The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. CONCLUSIONS: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Staphylococcus aureus
/
ADN Bacteriano
/
Reacción en Cadena de la Polimerasa
/
Técnicas Bacteriológicas
Tipo de estudio:
Estudio diagnóstico
/
Guía de Práctica Clínica
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
Medicina Tropical
Año:
2018
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de Minas Gerais/BR
/
Universidade Federal dos Vales do Jequitinhonha e Mucuri/BR
Similares
MEDLINE
...
LILACS
LIS