Comparison of methodologies for detecting Trypanosoma cruzi parasites by microscopic observation of microhematocrit capillary tubes
Rev. Soc. Bras. Med. Trop
;
52: e20180505, 2019. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1041570
ABSTRACT
Abstract INTRODUCTION: The microscopic examination of microhematocrit tubes (mHCT) has been proposed as the gold standard for acute and congenital Chagas disease diagnosis. We compared different mHCT methodologies detecting T. cruzi parasites in the blood. METHODS: The rotating method, water mount, and immersion oil methods were compared for their suitability, sensitivity, and specificity. RESULTS: The rotating method was easier, faster, and more sensitive than the others with 100% specificity. CONCLUSIONS: The rotating method is feasible for laboratory technicians with standard training in microscopic techniques and is recommended for the diagnosis of acute Chagas disease in primary health care facilities.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Trypanosoma cruzi
/
Centrifugación
/
Enfermedad de Chagas
/
Parasitemia
/
Tubo Capilar
/
Hematócrito
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
Medicina Tropical
Año:
2019
Tipo del documento:
Artículo
País de afiliación:
Bélgica
/
México
Institución/País de afiliación:
Universidad Autónoma de Yucatán/MX
/
Université Libre de Bruxelles/BE
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