Phage therapy as an approach to control salmonella enterica serotype enteritidis infection in mice
Rev. Soc. Bras. Med. Trop
;
52: e20190290, 2019. graf
Artículo
en Inglés
| LILACS
| ID: biblio-1057257
ABSTRACT
Abstract INTRODUCTION:
Salmonella enterica serotype Enteritidis (S. Enteritidis) is a cause of food-borne human illness. Given the prevalence of antibiotic resistance of Salmonella Enteritidis and the lack of antibiotic efficacy in future years, its replacement with other agents is necessary. One of the most useful agents is bacteriophages. METHODS S. Enteritidis was identified using a multiplex polymerase chain reaction assay. The effective bacteriophages were isolated from hospital wastewater samples. The effects of the bacteriophages were evaluated both in vitro and in vivo. RESULTS The phage SE20 belonged to the Podoviridae family, and the genome size was 40 kb. The evaluation of phage SE20 at variable pH ranges showed its susceptibility to pH < 3 and pH > 12. The animal model showed that mice infected with S. Enteritidis developed hepatomegaly and splenomegaly, but did not experience gastrointestinal complications after receiving the bacteriophages. CONCLUSIONS The results of this study suggest that phage SE20 is a promising candidate for controlling salmonellosis caused by Salmonella Enteritidis.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Salmonella enteritidis
/
Infecciones por Salmonella
/
Terapia de Fagos
Tipo de estudio:
Estudio pronóstico
/
Factores de riesgo
Límite:
Animales
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
Medicina Tropical
Año:
2019
Tipo del documento:
Artículo
País de afiliación:
Irán
Institución/País de afiliación:
School of Public Health (TUMS)/IR
/
Tehran University of Medical Sciences (TUMS)/IR
/
Tehran University of Medical Sciences/IR
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