Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae)
Rev. bras. entomol
;
63(4): 322-330, Out.-Dec. 2019. tab, graf
Artículo
en Inglés
|
LILACS-Express
| LILACS
| ID: biblio-1057790
ABSTRACT
ABSTRACT Wasps are a diverse group of insects that possess a sting apparatus associated with a venom gland, which is used for predation and colony defense. The biochemistry of Hymenoptera venom has been evaluated in relation to allergy and immunology, and proteomics has been shown to be a powerful tool for the identification of compounds with pharmacological potential. Data on wasps venom the of genus Apoica are scarce, so the objective of the present work was to identify the venom proteins of the eusocial wasp Apoica pallens, as a first step towards further investigation of applied uses of the venom and its protein constituents. The venom proteins were separated by two-dimensional gel electrophoresis, followed by MALDI-TOF/TOF mass spectrometry. A total of 259 spots were detected, with molecular weights from 4.9 to 141 kDa. Thirty of these proteins were identified and classified into eight functional categories allergen, enzyme, metabolism, structural, environmental response, proteoglycan, active in DNA and RNA, and unknown function. Due to the few available proteomic data for wasp venom, many proteins could not be identified, which makes studies with proteomic analysis of Hymenoptera venom even more important.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Idioma:
Inglés
Revista:
Rev. bras. entomol
Asunto de la revista:
Biologia
Año:
2019
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Estadual de Mato Grosso do Sul/BR
/
Universidade Federal da Grande Dourados/BR
/
Universidade Federal de Viçosa/BR
/
Universidade Federal do Maranhão/BR
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