Involvement of HSP90 in ischemic postconditioning-induced cardioprotection by inhibition of the complement system, JNK and inflammation
Acta cir. bras
;
35(1): e202000105, 2020. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1088523
ABSTRACT
Abstract Purpose To investigate whether heat shock protein 90 (HSP90) is involved in complement regulation in ischemic postconditioning (IPC). Methods The left coronary artery of rats underwent 30 min of occlusion, followed by 120 min of reperfusion and treatment with IPC via 3 cycles of 30s reperfusion and 30s occlusion. The rats were injected intraperitoneally with 1 mg/kg HSP90 inhibitor geldanamycin (GA) after anesthesia. Eighty rats were randomly divided into four groups: sham, ischemia-reperfusion (I/R), IPC and IPC + GA. Myocardial infarct size, apoptosis index and the expression of HSP90, C3, C5a, tumor necrosis factor (TNF)-alpha, interleukin (IL)-1β and c-Jun N-terminal kinase (JNK) were assessed. Results Compared with the I/R injury, the IPC treatment significantly reduced infarct size, release of troponin T, creatine kinase-MB, and lactate dehydrogenase, and cardiomyocyte apoptosis. These beneficial effects were accompanied by a decrease in TNF-α, IL-1β, C3, C5a and JNK expression levels. However, all these effects were abrogated by administration of the HSP90 inhibitor GA. Conclusion HSP90 exerts a profound effect on IPC cardioprotection, and may be linked to the inhibition of the complement system and JNK, ultimately attenuating I/R-induced myocardial injury and apoptosis.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Proteínas del Sistema Complemento
/
Daño por Reperfusión Miocárdica
/
Benzoquinonas
/
Proteínas HSP90 de Choque Térmico
/
Lactamas Macrocíclicas
/
Proteínas Quinasas JNK Activadas por Mitógenos
/
Infarto del Miocardio
Límite:
Animales
Idioma:
Inglés
Revista:
Acta cir. bras
Asunto de la revista:
Cirugía General
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Guang Xi Clinical Research Center for Cardiocerebrovascular Diseases/CN
/
Guang Xi Key Laboratory of Precision Medicine in Cardiocerebrovascular Diseases Control and Prevention/CN
/
Guang Xi Medical University/CN
Similares
MEDLINE
...
LILACS
LIS