Development of an enzyme-linked immunosorbent assay for diagnosis of human herpesvirus-7 infection
Rev. Soc. Bras. Med. Trop
;
53: e20190181, 2020. graf
Artículo
en Inglés
| LILACS
| ID: biblio-1092205
ABSTRACT
Abstract INTRODUCTION: Human herpesvirus (HHV)-7 establishes a latent infection during the lifetime of the host and can reactivate after the primary infection, leading to lytic replication in immunosuppressed patients. METHODS: This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) to identify HHV-7 serum antibodies and compare its performance with that of an indirect immunofluorescence assay (IFA). RESULTS: Serum samples (n=102) were tested by IgG-IFA and by ELISA. IFA and ELISA showed IgG-positive results in 77 and 73 samples, respectively. Qualitative concordance of 96% was demonstrated between the two techniques. CONCLUSIONS: ELISA may be useful to diagnose HHV-7 infection.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Inmunoglobulina G
/
Herpesvirus Humano 7
/
Infecciones por Roseolovirus
/
Anticuerpos Antivirales
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
/
Investigación cualitativa
Límite:
Humanos
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
Medicina Tropical
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Fundação Hermínio Ometto/BR
/
Instituto Butantan/BR
/
Universidade Federal dos Vales do Jequinhonha e Mucuri/BR
Similares
MEDLINE
...
LILACS
LIS