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Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours
Liver Research LaboratoryGrezzana Filho, Tomaz de Jesus Maria; Liver Research LaboratoryLongo, Larisse; Santos, Jorge Luiz dos; Liver Research LaboratoryGabiatti, Gemerson; Liver Research LaboratoryBoffil, Carlos; Liver Research LaboratorySantos, Emanuel Burck dos; Cerski, Carlos Thadeu Schmidt; Postgraduate Program in Surgical Sciences and Attending Liver Transplant SurgeonChedid, Marcio Fernandes; Division of General SurgeryCorso, Carlos Otavio.
  • Liver Research LaboratoryGrezzana Filho, Tomaz de Jesus Maria; Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre. Liver Research LaboratoryGrezzana Filho, Tomaz de Jesus Maria. Porto Alegre. BR
  • Liver Research LaboratoryLongo, Larisse; UFRGS. HCPA. Liver Research LaboratoryLongo, Larisse. Porto Alegre. BR
  • Santos, Jorge Luiz dos; University of Beira Interior. Health Science Research Centre. Covilhã. PT
  • Liver Research LaboratoryGabiatti, Gemerson; UFRGS. HCPA. Liver Research LaboratoryGabiatti, Gemerson. Porto Alegre. BR
  • Liver Research LaboratoryBoffil, Carlos; UFRGS. HCPA. Liver Research LaboratoryBoffil, Carlos. Porto Alegre. BR
  • Liver Research LaboratorySantos, Emanuel Burck dos; UFRGS. HCPA. Liver Research LaboratorySantos, Emanuel Burck dos. Porto Alegre. BR
  • Cerski, Carlos Thadeu Schmidt; UFRGS. HCPA. Porto Alegre. BR
  • Postgraduate Program in Surgical Sciences and Attending Liver Transplant SurgeonChedid, Marcio Fernandes; UFRGS. HCPA. Postgraduate Program in Surgical Sciences and Attending Liver Transplant SurgeonChedid, Marcio Fernandes. Porto Alegre. BR
  • Division of General SurgeryCorso, Carlos Otavio; UFRGS. HCPA. Division of General SurgeryCorso, Carlos Otavio. Porto Alegre. BR
Acta cir. bras ; 35(2): e202000205, 2020. graf
Artículo en Inglés | LILACS | ID: biblio-1130618
ABSTRACT
Purpose To investigate the effects of induction of selective liver hypothermia in a rodent model. Methods Seven male Wistar rats were subjected to 90 minutes of partial 70% liver ischemia and topic liver 26°C hypothermia (H group). Other seven male Wistar rats were subjected to 90 minutes of partial 70% normothermic liver ischemia (N group). Five additional rats underwent a midline incision and section of liver ligaments under normothermic conditions and without any liver ischemia (sham group). All animals were sacrificed 24-h after reperfusion, and livers were sampled for analyses. Pathology sections were scored for sinusoidal congestion, ballooning, hepatocelllular necrosis and the presence of neutrophilic infiltrates. Results At the end of the experiment, liver tissue expressions of TNF-ɑ, IL-1β, iNOS and TNF-ɑ/IL-10 ratio were significantly reduced in the H group compared to N group, whereas IL-10 and eNOS were significantly increased in H group. Histopathological injury scores revealed a significant decrease in ischemia/reperfusion (I/R) injuries in H group. Conclusion Selective liver hypothermia prevented I/R injury by inhibiting the release of inflammatory cytokines, preserves microcirculation, prevents hepatocellular necrosis and leukocyte infiltration, allowing maintenance of the liver architecture.
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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Daño por Reperfusión / Lesión Pulmonar Aguda / Hipotermia Inducida / Hígado Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Inglés Revista: Acta cir. bras Asunto de la revista: Cirugía General / Procedimentos Cir£rgicos Operat¢rios Año: 2020 Tipo del documento: Artículo País de afiliación: Brasil / Portugal Institución/País de afiliación: UFRGS/BR / Universidade Federal do Rio Grande do Sul/BR / University of Beira Interior/PT

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Daño por Reperfusión / Lesión Pulmonar Aguda / Hipotermia Inducida / Hígado Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Inglés Revista: Acta cir. bras Asunto de la revista: Cirugía General / Procedimentos Cir£rgicos Operat¢rios Año: 2020 Tipo del documento: Artículo País de afiliación: Brasil / Portugal Institución/País de afiliación: UFRGS/BR / Universidade Federal do Rio Grande do Sul/BR / University of Beira Interior/PT