Chronic alcoholism associated with diabetes induced apoptosis in the corpus cavernosum of rats
Acta cir. bras
;
35(8): e202000805, 2020. graf
Artículo
en Inglés
| LILACS
| ID: biblio-1130667
ABSTRACT
Abstract Purpose To evaluate the effects of alcohol exposure and diabetes on apoptotic process in the corpus cavernosum. Methods Forty eight male Wistar rats were divided into four groups control, diabetic, alcoholic and diabetic-alcoholic. Samples of the corpus cavernosum were prepared to study protein expression of apoptotic genes (Caspases-3 and 9) by immunohistochemistry and Real-Time PCR. Results The immunoreactivity of Caspases-3 and -9 was diffuse and higher in the treated groups though there was no significant difference between the experimental groups, only when compared with the control group. An increase was observed in the gene expression of Caspases-9 in the diabetic and ethanol-diabetic groups when compared with control and ethanol groups. Conclusions The association of these factors (ethanol and diabetes) probably can affect the apoptosis mechanism in lesions of the cavernous tissue in the rat penis. Both gene and protein expression of Caspase-9 in diabetic and ethanol-diabetic groups suggest the involvement of the apoptosis cascade from this study model.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Apoptosis
/
Alcoholismo
Tipo de estudio:
Estudio pronóstico
/
Factores de riesgo
Límite:
Animales
Idioma:
Inglés
Revista:
Acta cir. bras
Asunto de la revista:
Cirugía General
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
USP/BR
/
Universidade Estadual Paulista/BR
/
Universidade de Marília/BR
/
Universidade de São Paulo/BR
Similares
MEDLINE
...
LILACS
LIS