Biopolymer Xanthan: A New Adjuvant for DNA Vaccines
Braz. arch. biol. technol
;
63: e20190090, 2020. graf
Artículo
en Inglés
| LILACS
| ID: biblio-1132173
ABSTRACT
Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Polisacáridos Bacterianos
/
ADN Recombinante
/
Adyuvantes Inmunológicos
/
Xanthomonas campestris
/
Vacunas de ADN
Límite:
Animales
Idioma:
Inglés
Revista:
Braz. arch. biol. technol
Asunto de la revista:
Biologia
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Federal Rural University of Pernambuco/BR
/
Federal University of Pelotas/BR
Similares
MEDLINE
...
LILACS
LIS