Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study
Braz. arch. biol. technol
;
63: e20190179, 2020. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1132181
ABSTRACT
Abstract (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Tuberculosis Resistente a Múltiples Medicamentos
/
Antibióticos Antituberculosos
/
Mycobacterium tuberculosis
Tipo de estudio:
Ensayo Clínico Controlado
/
Estudio diagnóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Braz. arch. biol. technol
Asunto de la revista:
Biologia
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
Portugal
Institución/País de afiliación:
Adolfo Lutz Institute/BR
/
Federal University of Grande Dourados/BR
/
Federal University of Minas Gerais/BR
/
Federal University of Rio Grande/BR
/
Federal University of Rio de Janeiro/BR
/
NOVA University of Lisbon/PT
/
Oswaldo Cruz Foundation/BR
/
Sergio Arouca National Public Health School/BR
/
State Secretariat of Health of Rio Grande do Sul/BR
/
Tropical Medicine Foundation Dr. Heitor Vieira Dourado/BR
Similares
MEDLINE
...
LILACS
LIS