Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus Paracoccidioides
J. venom. anim. toxins incl. trop. dis
;
26: e20200023, 2020. tab, graf, ilus
Artículo
en Inglés
| LILACS, VETINDEX
| ID: biblio-1135136
ABSTRACT
Endemic systemic mycoses remain a health challenge, since these opportunistic diseases are increasingly infecting immunosuppressed patients. The simultaneous use of antifungal compounds and other drugs to treat infectious or non-infectious diseases has led to several interactions and undesirable effects. Thus, new antifungal compounds should be investigated. The present study aimed to evaluate the activity of liriodenine extracted from Annona macroprophyllata on agents of systemic mycoses, with emphasis on the genus Paracoccidioides. Methods:
The minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined by the microdilution method. The cellular alterations caused by liriodenine on a standard P. brasiliensis (Pb18) strain were evaluated by transmission and scanning electron microscopy.Results:
Liriodenine was effective only in 3 of the 8 strains of the genus Paracoccidioides and in the Histoplasma capsulatum strain, in a very low concentration (MIC of 1.95 µg.mL-1); on yeasts of Candida spp. (MIC of 125 to 250 µg.mL-1), including C. krusei (250 µg.mL-1), which has intrinsic resistance to fluconazole; and in Cryptococcus neoformans and Cryptococcus gattii (MIC of 62.5 µg.mL-1). However, liriodenine was not effective against Aspergillus fumigatus at the studied concentrations. Liriodenine exhibited fungicidal activity against all standard strains and clinical isolates that showed to be susceptible by in vitro tests. Electron microscopy revealed cytoplasmic alterations and damage to the cell wall of P. brasiliensis (Pb18).Conclusion:
Our results indicate that liriodenine is a promising fungicidal compound that should undergo further investigation with some chemical modifications.(AU)
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Paracoccidioides
/
Microscopía Electrónica
/
Pruebas de Sensibilidad Microbiana
/
Cryptococcus neoformans
/
Cryptococcus gattii
/
Micosis
Idioma:
Inglés
Revista:
J. venom. anim. toxins incl. trop. dis
Año:
2020
Tipo del documento:
Artículo
Institución/País de afiliación:
São Paulo State University/BR
/
Universidad de Ciencias y Artes de Chiapas/MX
/
Universidade do Oeste Paulista/BR
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