Latex proteins downregulate inflammation and restores blood-coagulation homeostasis in acute Salmonella infection
Mem. Inst. Oswaldo Cruz
;
115: e200458, 2020. graf
Artículo
en Inglés
| LILACS, SES-SP
| ID: biblio-1135229
ABSTRACT
BACKGROUND Calotropis procera latex protein fraction (LP) was previously shown to protect animals from septic shock. Further investigations showed that LP modulate nitric oxide and cytokines levels. OBJECTIVES To evaluate whether the protective effects of LP, against lethal bacterial infection, is observed in its subfractions (LPPII and LPPIII). METHODS Subfractions (5 and 10 mg/kg) were tested by i.p. administration, 24 h before challenging with lethal injection (i.p.) of Salmonella Typhimurium. LPPIII (5 mg/kg) which showed higher survival rate was assayed to evaluate bacterial clearance, histopathology, leukocyte recruitment, plasma coagulation time, cytokines and NO levels. FINDINGS LPPIII protected 70% of animals of death. The animals given LPPIII exhibited reduced bacterial load in blood and peritoneal fluid after 24 h compared to the control. LPPIII promoted macrophage infiltration in spleen and liver. LPPIII restored the coagulation time of infected animals, increased IL-10 and reduced NO in blood. MAIN CONCLUSIONS LPPIII recruited macrophages to the target organs of bacterial infection. This addressed inflammatory stimulus seems to reduce bacterial colonisation in spleen and liver, down regulate bacterial spread and contribute to avoid septic shock.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Proteínas de Plantas
/
Infecciones por Salmonella
/
Extractos Vegetales
/
Calotropis
/
Homeostasis
/
Inflamación
/
Látex
/
Antibacterianos
Tipo de estudio:
Estudios de evaluación
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Año:
2020
Tipo del documento:
Artículo
Institución/País de afiliación:
Universidade Federal Rural de Pernambuco/BR
/
Universidade Federal do Ceará/BR
/
Universidade de Fortaleza/BR
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