Lipopolysaccharides' Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells
Pesqui. bras. odontopediatria clín. integr
;
20: e0048, 2020. tab, graf
Artículo
en Inglés
| LILACS, BBO
| ID: biblio-1135491
ABSTRACT
Abstract Objective:
To show the cytotoxicity of Porphyromonas gingivalis lipopolysaccharide (LPS) on human umbilical cord mesenchymal stem cells (HUCMSCs) to better understand the characteristics for its application in regenerative procedures under periodontopathogen LPS influence. Material andMethods:
Ultrapure Porphyromonas gingivalis LPS was used in this study. This research used a frozen stock HUCMSCs, previously confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSCs were cultured and divided into two groups, the control group and LPS group with various concentrations from 25 to 0.39 µg/mL. MTT assay was done and the cells were observed and counted. The significance level was set at 5%.Results:
The percentage of living HUCMSCs on LPS group were not significantly different among concentrations (p>0.05) from 25 to 0.39 µg/mL, even though there were slight mean decrease between groups, but they were not significant. The duration of 24 hours of exposure of LPS does not significantly lower HUCMSCs viability.Conclusion:
LPS does not affect the viability of HUCMSCs. The lower the concentration of LPS, the higher the viability of HUCMSCs.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Cordón Umbilical
/
Lipopolisacáridos
/
Porphyromonas gingivalis
/
Citotoxicidad Inmunológica
/
Células Madre Mesenquimatosas
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Pesqui. bras. odontopediatria clín. integr
Asunto de la revista:
Odontología
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
Indonesia
Institución/País de afiliación:
Universitas Airlangga/ID
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