Differences in Proteomic Profiles Between Caries Free and Caries Affected Children
Pesqui. bras. odontopediatria clín. integr
;
20: e5592, 2020. tab
Artículo
en Inglés
| LILACS, BBO
| ID: biblio-1135503
ABSTRACT
Abstract Objective:
To determine if there are differences in protein profiles in saliva depending if children of caries-free versus caries affected. Material andMethods:
A cohort of 91 children with ages between 6 and 19 years, along clinical status of caries experience. Protein profiles in saliva were determined using electrophoresis and the calculation of the percentage of a specific band at a specific molecular weight in relationship to the total protein in that sample (% of total) using molecular weight standards. This quantification was repeated for each protein band across a range of molecular weights for each sample. Chi-square, Fisher's exact, and Student t-tests were used to compare the distributions between caries-free and caries affected children (α=0.05).Results:
Histatin was more likely to be non-detectable or reduced in caries-free children (OR=7.56; 95% CI 1.62-35.13) and these children had on average one less gel band detected by the assay we used.Conclusion:
We have found differences in proteins between caries affected and caries-free children, suggesting that this line of investigation holds the promise of providing new tools for caries management.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Saliva
/
Caries Dental
/
Proteómica
/
Electroforesis
Tipo de estudio:
Guía de Práctica Clínica
Límite:
Adolescente
/
Adulto
/
Niño
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
America del Norte
Idioma:
Inglés
Revista:
Pesqui. bras. odontopediatria clín. integr
Asunto de la revista:
Odontología
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
Estados Unidos
Institución/País de afiliación:
University of Arizona/US
/
University of Pittsburgh/US
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