Risk Factors for Early Childhood Caries Based on Identification of Veillonella spp. Using RT-PCR
Pesqui. bras. odontopediatria clín. integr
;
20: e5701, 2020. tab
Artículo
en Inglés
| LILACS, BBO
| ID: biblio-1135530
ABSTRACT
Abstract Objective:
To identify the occurrence of Veillonella spp. in children using real-time PCR (RT-PCR) and to determine its role as a risk factor for ECC in children aged 2-3 years. Material andMethods:
A cross-sectional survey was conducted and samples from 87 children aged 2-3 years, who lived in selected villages in the Bandung City area, Indonesia, were collected. Examination for dental caries was performed using standard checks for decay, missing, and filled surfaces (dfms), and saliva samples were taken. Microbiological examination was performed using RT-PCR with primers consisting of one primary set for Veillonella spp. and one universal primary set for 16S rDNA. We performed statistical testing using the Mann Whitney rank-sum test.Results:
A total of 87 children were sampled, and an ECC prevalence of 71.3% was found, with a mean dmfs of 7.1 (± 9.1). The proportion of Veillonella spp. in caries-free children was 2.13 ± 2.30, while in children with ECC, it was 3.29 ± 6.83.Conclusion:
The proportion of Veillonella spp. in children with ECC was higher than in caries-free children; therefore, Veillonella spp. may be a risk factor for ECC.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Veillonella
/
Reacción en Cadena de la Polimerasa
/
Factores de Riesgo
/
Caries Dental
/
Bacterias Gramnegativas
Tipo de estudio:
Estudio diagnóstico
/
Estudio de etiología
/
Estudio observacional
/
Estudio de prevalencia
/
Estudio pronóstico
/
Factores de riesgo
Límite:
Child, preschool
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Pesqui. bras. odontopediatria clín. integr
Asunto de la revista:
Odontología
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
Indonesia
Institución/País de afiliación:
University of Indonesia/ID
/
University of Padjadjaran/ID
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