In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil
Rev. Soc. Bras. Med. Trop
;
53: e20200657, 2020. tab, graf
Artículo
en Inglés
| SES-SP, ColecionaSUS, LILACS
| ID: biblio-1143868
ABSTRACT
Abstract INTRODUCTION:
Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results.METHODS:
Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays.RESULTS:
Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences.CONCLUSION:
These results should be taken into consideration when using PCR-based diagnostics in Brazil.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Genoma Viral
/
Infecciones por Coronavirus
/
Betacoronavirus
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Año:
2020
Tipo del documento:
Artículo
Institución/País de afiliación:
Universidade de Fortaleza/BR
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