Development and validation of an UV-Vis spectrophotometric method for the quantification of oclacitinib in capsule formulation
Rev. ciênc. farm. básica apl
;
42: 8, 20210101.
Artículo
en Inglés
|
LILACS-Express
| LILACS
| ID: biblio-1148221
ABSTRACT
Objective. The aim of this study was the development and validation of an UV-Vis spectrophotometric method for the quantification of oclacitinib in commercial capsule formulation since pharmacopeias have not yet provided an official monograph for this drug. Methods. The parameters linearity, limit of detection, limit of quantitation, specificity, precision, accuracy, and robustness were determined according to Brazilian and international guidelines. Results. Linearity was determined for the analytical range of 5-15 µg/mL, and a limit of detection of 1.18 µg/mL and limit of quantification of 3.58 µg/mL were obtained. The method was selective and the precision was demonstrated through repeatability and intermediate precision, with relative standard deviations of 1.96% and 1.78%, respectively. In its turn, accuracy presented recovery percentages of 98.32-100.91%. All robustness and sample stability (48 h at 25 °C) results revealed no statistical variation among the groups. Conclusions. The presented method is suitable for the quantification of oclacitinib in commercial capsule formulation.
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Índice:
LILACS (Américas)
Idioma:
Inglés
Revista:
Rev. ciênc. farm. básica apl
Asunto de la revista:
Farmacología
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Departamento de Farmácia/BR
/
Programa de Pós-graduação em Ciências Farmacêuticas/BR
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