Impact of cell harvesting methods on detection of cell surface proteins and apoptotic markers
Braz. j. med. biol. res
;
54(2): e10197, 2021. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1153509
ABSTRACT
Assays based on the flow cytometry technique allow a convenient analysis of multiple cellular parameters; however, their results should be interpreted cautiously due to a strong impact of confounding factors. Different techniques in cell culturing such as either enzymatic or mechanic detachment of adherent cells can heavily influence the structure of the cell membrane or presence of the surface antigens leading to strong false positive signals, and finally, substantial experimental bias. The aim of our study was to assess and compare the impact of cell harvesting methods (both enzymatic and non-enzymatic) on the apoptosis process and on the surface antigen cytometric analyses. We found significant differences in the quality of analysis in terms of the amount of detected surface markers determined by the detachment method. Our results demonstrated clearly how important it is to carefully choose the appropriate detachment method and may help to avoid mistakes in experiment planning. In conclusion, we recommend to adjust the detachment method to the type of analyzed markers (surface antigens or translocated phosphatidylserine).
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Separación Celular
/
Apoptosis
/
Proteínas de la Membrana
/
Antígenos de Superficie
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
Polonia
Institución/País de afiliación:
Institute of Bioorganic Chemistry, Polish Academy of Sciences/PL
/
University of Life Sciences/PL
Similares
MEDLINE
...
LILACS
LIS