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Desarrollo de un antígeno para diagnóstico del parvovirus de las ratas (virus Kilham) por la técnica de inhibición de la hemaglutinación / [Development of an antigen for the diagnosis of Kilham rat parvovirus by hemagglutination inhibition test]
Ayala, M A; Laborde, J; Milocco, S; Carbone, C; Cid de la Paz, V; Galosi, C M.
  • Ayala, M A; s.af
  • Laborde, J; s.af
  • Milocco, S; s.af
  • Carbone, C; s.af
  • Cid de la Paz, V; s.af
  • Galosi, C M; Cátedra de Animales de Laboratorio,, Facultad de Ciencias Veterinarias, UNLP, Calle 60 y 118, 1900 La Plata, Argentina..
Rev. argent. microbiol ; 36(1): 16-9, Jan.-Mar. 2004.
Artículo en Español | LILACS-Express | LILACS, BINACIS | ID: biblio-1171742
ABSTRACT
An antigen of rat parvovirus (Kilham virus) was developed for the diagnosis of viral infection in rat colonies by using hemagglutination inhibition (HAI) test. Primary cell cultures from rat embryos were infected with Kilham rat virus. Infected cells obtained at different time post infection were scraped, centrifuged, concentrated one hundred times, sonicated and centrifuged again. The supernatants obtained were titrated by hemagglutination. The specificity was confirmed with positive and negative reference sera. Ninety eight serum samples were studied by using HAI test. The results coincided with those obtained in a reference laboratory. Kilham rat parvovirus antigen obtained from 5 days-infected-cells was specific, sensitive, easy to prepare, with a high yield and it is useful to detect this virus in experimental and production rat colonies.
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Índice: LILACS (Américas) Tipo de estudio: Estudio diagnóstico Idioma: Español Revista: Rev. argent. microbiol Asunto de la revista: Microbiologia Año: 2004 Tipo del documento: Artículo

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Índice: LILACS (Américas) Tipo de estudio: Estudio diagnóstico Idioma: Español Revista: Rev. argent. microbiol Asunto de la revista: Microbiologia Año: 2004 Tipo del documento: Artículo